Mass spectrometric analysis of combinatorial peptide libraries derived from the tandem repeat unit of MUC2 mucin.
Identifieur interne : 003210 ( Main/Exploration ); précédent : 003209; suivant : 003211Mass spectrometric analysis of combinatorial peptide libraries derived from the tandem repeat unit of MUC2 mucin.
Auteurs : Gitta Schlosser [Hongrie] ; Zoltán Takáts ; Károly Vékey ; Gabriella P Csfalvi ; Antonio Malorni ; Emöke Windberg ; Andrea Kiss ; Ferenc HudeczSource :
- Journal of peptide science : an official publication of the European Peptide Society [ 1075-2617 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Mucins.
- chemical , chemistry : Mucins.
- methods : Mass Spectrometry.
- Amino Acid Sequence, Combinatorial Chemistry Techniques, Humans, Molecular Sequence Data, Peptide Library, Repetitive Sequences, Amino Acid, Time Factors.
Abstract
Four 19-member synthetic peptide libraries, based on the TX1TX2T epitope motif of the mucin-2 gastrointestinal glycoprotein (MUC2) and ranging in peptide length from dipeptides to 15-mers (XT, TXT, TQTXT and KVTPTPTPTGTQTXT), were synthesized by combinatorial solid phase peptide synthesis using the portioning-mixing combinatorial approach, and analysed by electrospray ionization mass spectrometry at different (1000-10000) resolutions. Most of the components of the individual libraries could be easily identified in a single-stage molecular mass screening experiment. The resolving power of the instrument becomes an important factor above 800-1000 Da molecular mass, when predominantly multiply charged molecular ions are formed. Approaches to the identification of isobars (glutamine/lysine), isomers leucine/isoleucine) and sequence variations by tandem mass spectrometry, and/or by high-performance liquid chromatography-mass spectrometry are outlined.
DOI: 10.1002/psc.462
PubMed: 12846482
Affiliations:
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Vékey</name></author><author><name sortKey="P Csfalvi, Gabriella" sort="P Csfalvi, Gabriella" uniqKey="P Csfalvi G" first="Gabriella" last="P Csfalvi">Gabriella P Csfalvi</name></author><author><name sortKey="Malorni, Antonio" sort="Malorni, Antonio" uniqKey="Malorni A" first="Antonio" last="Malorni">Antonio Malorni</name></author><author><name sortKey="Windberg, Emoke" sort="Windberg, Emoke" uniqKey="Windberg E" first="Emöke" last="Windberg">Emöke Windberg</name></author><author><name sortKey="Kiss, Andrea" sort="Kiss, Andrea" uniqKey="Kiss A" first="Andrea" last="Kiss">Andrea Kiss</name></author><author><name sortKey="Hudecz, Ferenc" sort="Hudecz, Ferenc" uniqKey="Hudecz F" first="Ferenc" last="Hudecz">Ferenc Hudecz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12846482</idno><idno type="pmid">12846482</idno><idno type="doi">10.1002/psc.462</idno><idno 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of MUC2 mucin.</title><author><name sortKey="Schlosser, Gitta" sort="Schlosser, Gitta" uniqKey="Schlosser G" first="Gitta" last="Schlosser">Gitta Schlosser</name><affiliation wicri:level="3"><nlm:affiliation>Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Chemical Research Center, Hungarian Academy of Sciences, Budapest</wicri:regionArea><placeName><settlement type="city">Budapest</settlement><region nuts="2">Hongrie centrale</region></placeName></affiliation></author><author><name sortKey="Takats, Zoltan" sort="Takats, Zoltan" uniqKey="Takats Z" first="Zoltán" last="Takáts">Zoltán Takáts</name></author><author><name sortKey="Vekey, Karoly" sort="Vekey, Karoly" uniqKey="Vekey K" first="Károly" last="Vékey">Károly Vékey</name></author><author><name sortKey="P Csfalvi, Gabriella" sort="P Csfalvi, Gabriella" uniqKey="P Csfalvi G" first="Gabriella" last="P Csfalvi">Gabriella P Csfalvi</name></author><author><name sortKey="Malorni, Antonio" sort="Malorni, Antonio" uniqKey="Malorni A" first="Antonio" last="Malorni">Antonio Malorni</name></author><author><name sortKey="Windberg, Emoke" sort="Windberg, Emoke" uniqKey="Windberg E" first="Emöke" last="Windberg">Emöke Windberg</name></author><author><name sortKey="Kiss, Andrea" sort="Kiss, Andrea" uniqKey="Kiss A" first="Andrea" last="Kiss">Andrea Kiss</name></author><author><name sortKey="Hudecz, Ferenc" sort="Hudecz, Ferenc" uniqKey="Hudecz F" first="Ferenc" last="Hudecz">Ferenc Hudecz</name></author></analytic><series><title level="j">Journal of peptide science : an official publication of the European Peptide Society</title><idno type="ISSN">1075-2617</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Combinatorial Chemistry Techniques</term><term>Humans</term><term>Mass Spectrometry (methods)</term><term>Molecular Sequence Data</term><term>Mucins (analysis)</term><term>Mucins (chemistry)</term><term>Peptide Library</term><term>Repetitive Sequences, Amino Acid</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Humains</term><term>Mucines ()</term><term>Mucines (analyse)</term><term>Spectrométrie de masse ()</term><term>Séquence d'acides aminés</term><term>Séquences répétées d'acides aminés</term><term>Techniques de chimie combinatoire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Mucins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Mucins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Mucines</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Combinatorial Chemistry Techniques</term><term>Humans</term><term>Molecular Sequence Data</term><term>Peptide Library</term><term>Repetitive Sequences, Amino Acid</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Banque de peptides</term><term>Données de séquences moléculaires</term><term>Facteurs temps</term><term>Humains</term><term>Mucines</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term><term>Séquences répétées d'acides aminés</term><term>Techniques de chimie combinatoire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four 19-member synthetic peptide libraries, based on the TX1TX2T epitope motif of the mucin-2 gastrointestinal glycoprotein (MUC2) and ranging in peptide length from dipeptides to 15-mers (XT, TXT, TQTXT and KVTPTPTPTGTQTXT), were synthesized by combinatorial solid phase peptide synthesis using the portioning-mixing combinatorial approach, and analysed by electrospray ionization mass spectrometry at different (1000-10000) resolutions. Most of the components of the individual libraries could be easily identified in a single-stage molecular mass screening experiment. The resolving power of the instrument becomes an important factor above 800-1000 Da molecular mass, when predominantly multiply charged molecular ions are formed. Approaches to the identification of isobars (glutamine/lysine), isomers leucine/isoleucine) and sequence variations by tandem mass spectrometry, and/or by high-performance liquid chromatography-mass spectrometry are outlined.</div></front></TEI><affiliations><list><country><li>Hongrie</li></country><region><li>Hongrie centrale</li></region><settlement><li>Budapest</li></settlement></list><tree><noCountry><name sortKey="Hudecz, Ferenc" sort="Hudecz, Ferenc" uniqKey="Hudecz F" first="Ferenc" last="Hudecz">Ferenc Hudecz</name><name sortKey="Kiss, Andrea" sort="Kiss, Andrea" uniqKey="Kiss A" first="Andrea" last="Kiss">Andrea Kiss</name><name sortKey="Malorni, Antonio" sort="Malorni, Antonio" uniqKey="Malorni A" first="Antonio" last="Malorni">Antonio Malorni</name><name sortKey="P Csfalvi, Gabriella" sort="P Csfalvi, Gabriella" uniqKey="P Csfalvi G" first="Gabriella" last="P Csfalvi">Gabriella P Csfalvi</name><name sortKey="Takats, Zoltan" sort="Takats, Zoltan" uniqKey="Takats Z" first="Zoltán" last="Takáts">Zoltán Takáts</name><name sortKey="Vekey, Karoly" sort="Vekey, Karoly" uniqKey="Vekey K" first="Károly" last="Vékey">Károly Vékey</name><name sortKey="Windberg, Emoke" sort="Windberg, Emoke" uniqKey="Windberg E" first="Emöke" last="Windberg">Emöke Windberg</name></noCountry><country name="Hongrie"><region name="Hongrie centrale"><name sortKey="Schlosser, Gitta" sort="Schlosser, Gitta" uniqKey="Schlosser G" first="Gitta" last="Schlosser">Gitta Schlosser</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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